An infected triatomine insect vector takes a blood meal and releases trypomastigotes in its feces near the site of the bite wound. After metacyclic trypomastigotes pass through the skin, they briefly travel in the blood stream and then colonize muscle and neuron tissues, where they differentiate into intracellular amastigotes. The amastigotes are released into the circulation as bloodstream trypomastigotes. When triatomine insect feeds on blood from an infected animal or human, they ingest trypomastigotes. These trypomastigotes turn into epimastigotes in the vector’s midgut where the parasite proliferates and transforms to metacyclic trypomastigotes that will be released in the feces of the insect.