SPR Unit

SPR Unit

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The SPR unit provides a service to scientist from the IQM, other CSIC institutes or external centers as public research organisms, universities or private companies. The unit is equipped with a Biacore X-100 technology which allows to detect interactions in real time between two or more biomolecules without any kind of label. The specificity of the interactions or kinetic and affinity parameters are the main information which can be obtain of a SPR experiment. The data analysis provides the rate constants and the affinity constant of the complex (KD, kon and koff).

Besides, it can be useful for determining thermodynamical parameters through the measurement at different temperatures.

The high sensibility of this technique is the main advantage, fundamental in the drug- receptor interactions. The different samples which can be analyzed are biomolecules (proteins, peptides, lipids..), drugs, or organic and inorganic compounds.

For further information about this technology:      

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APPLICATIONS

 

  • Drug-receptor interaction
  • Protein-protein interaction
  • Protein-Carbohydrated interaction
  • Protein-Lipid interaction
  • Enzyme-substrate interaction
  • Antigen-antibody interaction
  • Drug design (characterization of modified compounds)
  • DNA-protein and DNA-small molecule interaction

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EQUIPMENT

 

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PUBLICATIONS

 

  • Morales, P.; Gómez-Cañas, M.;, Navarro, G.; Hurst, D.P.; Carrillo-Salinas, F.J.; Lagartera, L.; Pazos, R.; Goya, P.; Reggio, P.H.; Guaza, C.; Franco, R.; Fernández-Ruiz, J.; Jagerovic, N. (2016) Chromenopyrazole, a Versatile Cannabinoid Scaffold with in Vivo Activity in a Model of Multiple Sclerosis. J Med Chem. 59:6753-71.

  • Ríos Martínez, C.H.; Miller, F.; Ganeshamoorthy, K.; Glacial, F.; Kaiser, M.; de Koning, H.P.; Eze, A.A.; Lagartera, L.; Herraiz, T.; Dardonville, C. (2015) A new nonpolar N-hydroxy imidazoline lead compound with improved activity in a murine model of late-stage Trypanosoma brucei brucei infection is not cross-resistant with diamidines. Antimicrob Agents Chemother. 59:890-904.

  • Ríos Martínez, C.H.; Lagartera, L.; Trujillo, C.; Dardonville, C. (2015) Bisimidazoline arylamides binding to the DNA minor groove: N1-hydroxylation enhances binding affinity and selectivity to AATT sites. Med. Chem. Commun. 6: 2036-2042.

  • Ríos Martínez, C.H.; Lagartera, L.; Kaiser, M.; Dardonville, C. (2014) Antiprotozoal activity and DNA binding of N-substituted N-phenylbenzamide and 1,3-diphenylurea bisguanidines. Eur J Med Chem. 23; 81:481-91.

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RATES

 

These rates offer the following support:

  • Experimental design
  • Acquisition of experimental data
  • Preparation of necessary buffers for measurement
  • Provision of experimental data as exportable computer files
  • Brief report about the interactions and, where appropiate, speed and association/dissociation rates

The detailed analysis, preparation of figures and/or texts for publications are not included in the service. According to each situation, the conditions for the inclusion in the “colaboration” mode will be negociated. .

CONTACT

 

For any information about SPR unit:

[box type=”info”]Dra. Laura Lagartera Ortiz

l.lagartera@iqm.csic.es

Telephone number: 915622900    Ext 356[/box]

 

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LINKS

 

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